منابع مشابه
Reconstitution of delipidated bacteriorhodopsin with endogenous polar lipids.
Delipidated bacteriorhodopsin has been reconstituted with endogenous polar lipids from Halobacterium halobium. The vesicle (diameter, 250-500 A) formed are very stable, relatively homogeneous in bacteriorhodopsin and lipid content, and almost optically clear; a minor turbid fraction can be separated by gel filtration. Bacteriorhodopsin in the reconstituted vesicles has an inside out orientation...
متن کاملReconstitution of bacteriorhodopsin vesicles with Halobacterium halobium lipids. Effects of variations in lipid composition.
Reconstitution of bacteriorhodopsin into vesicles has been studied using individual and defined mixtures of fractionated Halobacterium halobium polar lipids. The lipids varied greatly in their ability to form vesicles that translocated protons and showed stimulation by valinomycin. Thus, phosphatidylglycerol phosphate gave mainly lipid-protein aggregates, while glycolipid sulfate gave mainly fu...
متن کاملRefolding of an integral membrane protein. Denaturation, renaturation, and reconstitution of intact bacteriorhodopsin and two proteolytic fragments.
The complete denaturation and subsequent renaturation and reconstitution of a polytopic integral membrane protein are demonstrated. Delipidated bacteriorhodopsin (Huang, K.-S., Bayley, H., and Khorana, H. G. (1980) Proc. Natl. Acad. Sci. U. S. A. 77, 323-327) is completely denatured when transferred into 88% formic acid or anhydrous trifluoroacetic acid as shown by NMR and circular dichroism sp...
متن کاملReconstitution of Sand Specimens Using a Rainer System
Air pluviation is a method of preparing laboratory models to carry out many geotechnical experiments. In this study, a new air pluviation device has been developed by the researchers. For laboratory modeling, it is important to calibrate the device using air pluviation to produce accurate specimens with desirable conditions and relative densities. A series of laboratory tests were conducted to ...
متن کاملPotential applications of bacteriorhodopsin mutants.
Bacteriorhodopsin (BR), a model system in biotechnology, is a G-protein dependent trans membrane protein which serves as a light driven proton pump in the cell membrane of Halobacterium salinarum. Due to the linkage of retinal to the protein, it seems colored and has numbers of versatile properties. As in vitro culture of the Halobacteria is very difficult, and isolation is time consuming and u...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1974
ISSN: 0014-5793
DOI: 10.1016/0014-5793(74)81153-1